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Membrane polarisation assays of compounds 1 and 37a against SA-1199B at three different concentrations (1, 5 and 10 µg/mL) using the BacLight Bacterial Membrane Potential Kit. CCCP was used as positive control at 1 µg/mL (5 µM). % of membrane polarisation was calculated from the red/green fluorescence ratio by comparing bacterial cells in the presence of compounds with untreated cells.
