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. 2020 Feb 14;11:218. doi: 10.3389/fmicb.2020.00218

FIGURE 3.

FIGURE 3

The effects of pH and temperature on the depolymerase activity. The EPS of K. pneumoniae 94 and 256 was mixed with Dpo42 (A) and Dpo43 (C), respectively, with pH adjusted with 100 mM citric acid-Na2HPO4 buffer (pH 3.0–8.0) or 100 mM Glycine-NaOH buffer (pH 9.0–10.0) and incubated for 1 h at 37°C. The EPS of K. pneumoniae 94 and 256 was incubated with Dpo42 (B) and Dpo43 (D), respectively, with pH adjusted to 5 and temperature varied between 20–90°C for 1 h. EPS-alone in citric acid-Na2HPO4 buffer (pH 5.0) at RT served as a control. The CPC was added to the mixture for 5 min incubation at RT. The turbidity of residual EPS was determined at 600 nm. Data are expressed as means ± SD (n = 3).