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. 2020 Dec 13;69(3):342–354. doi: 10.2337/db19-0281

Figure 2.

Figure 2

Relatively few LDs are present in the juvenile human pancreas. A: Pancreatic LDs are difficult to detect by Nile Red neutral lipid staining in juvenile (11 months old [11m] to 7 years old [7yr]) in comparison with teenage (19 years old) and adult (50 years old) human donors. The islet hormone area is depicted in green and LDs (Nile red) are depicted in red and nuclei in blue (DAPI). Scale bar = 50 μm. B: Percent of the islet LD area in the various age-groups (n = 6–8 donors/group). yr, years. C: The percent of LD containing islets in the donor age-groups. All error bars indicate SEM. **P < 0.01 and ***P < 0.001 vs. 0- to 2-year-old group; $$P < 0.01 and $$$P < 0.001 vs. 3- to 11-year-old group; +++P < 0.001 vs. 16- to 20-year-old group.