Figure 3.

Intrinsic human islet factors determine the LD levels. A: Workflow schematic of the healthy juvenile and adult islet transplantation studies. Islets were transplanted under the kidney capsule for 6 weeks. B: Representative images of juvenile and adult islets showing that LD number is much higher in adult transplanted islets. The islet hormone area is depicted in green and LDs are depicted (Nile red) in red and nuclei (DAPI) in blue. Scale bar = 50 μm. C: Quantification of the percent of LD⁺ cells in the transplanted hormone⁺ cell area ± SEM. m, months old; Tx, transplant; yr, years old.