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. 2020 Feb 14;11:80. doi: 10.3389/fimmu.2020.00080

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Copyright © 2020 Mehanny, Koch, Lehr and Fuhrmann.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Viability assessment of dendritic cells (DC2.4) after exposure to pneumococcal membrane vesicles (MVs) by flow cytometry. (A) Overlay of recorded fluorescence intensity of phycoerythrin (PE-A) channel, after exposure of DC2.4 cells to various concentrations of MVs, in addition to live and dead cell controls. (B) Calculated percentage viability of DC2.4 cells with respect to cells treated with PBS (live cells control) and 4% PFA (dead cells control), after exposure to streptococcal MVs. The dashed line separates PE-A channel negative and positive cells. n = 3, mean ± SD.