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. 2020 Feb 14;11:104. doi: 10.3389/fimmu.2020.00104

Figure 2.

Figure 2

Flow cytometry analysis of TFH subset levels and cytokine secretion in UC patients. (A,B) Peripheral blood from active UC patients (n = 32), stable remission UC patients (n = 32), and HC (n = 32) was collected and TFH subsets were analyzed. TFH1, TFH2, and TFH17 were defined as follows: TFH1 (CD3+CD4+CXCR5+FoxP3−CXCR3+CCR6−), TFH2 (CD3+CD4+CXCR5+FoxP3−CXCR3−CCR6−), and TFH17 (CD3+CD4+CXCR5+FoxP3−CXCR3− CCR6+). (A) Representative cytometry strategy for detection of CXCR3 and CCR6 expressions in CD3+CD4+CXCR5+ FoxP3− T cells. Numbers indicate the percentage of cells in each quadrant. (B) The comparison of average percentage (up) and absolute number per liter (bottom) of TFH1, TFH2, TFH17 subsets among active UC patients, patients in stable remission UC and HC. (C) Peripheral blood from active UC patients (n = 18), stable remission UC patients (n = 18) and HC (n = 16) were incubated with PMA, ionomycin and BFA for 5 h and cytokines within TFH subsets were analyzed by intracellular staining. Representative dot plots of IL-4 secretion levels in TFH2 subset and IL-17A secretion levels in TFH17 subset. Numbers show the percentages of IL-4+ cells among TFH2 and IL-17A+ cells among TFH17 lymphocytes (up). IL-4+ cell percentages in TFH2 and IL-17A+ cells among TFH17 were compared (bottom), respectively. All Symbols represent individuals and bars show the mean ± SD. *p < 0.05; ***p < 0.001; ns, not significant.