Figure 3. 3D antibiotic scaffolds are not cytotoxic.
Primary human osteoblasts were cultured on 3D antibiotic (Abx) scaffolds in vitro for 7 or 14 days, whereupon (A) osteoblast viability was examined by confocal microscopy and (B) cell survival was demonstrated by MTT assay. (C) Survival of primary mouse neuron-glial cultures after exposure to conditioned medium from 3D antibiotic scaffolds incubated for 1–14 days in medium as assessed by LDH release assays. Results are reported as the mean ± SD of three biological replicates.