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. 2020 Feb 12;26:e918933-1–e918933-11. doi: 10.12659/MSM.918933

Figure 4.

Figure 4

Inhibition of oxidative stress and expression of inflammatory cytokines was accompanied by activation of SIRT1-FOXO1-p53 signaling. (A) Western blot results of SIRT1, FOXO1, and p53 expression in human cardiomyocytes (AC16) with or without silencing of IL-36R in vitro. (B) Representative figures of ROS production evaluated by DCFA-FH fluorescent probes in the human AC16 cell line with or without silencing of IL-36R in vitro. (C) Quantitative analysis of relative fluorescent intensity of DCFA-FH probes in AC16 with or without silencing of IL-36R in vitro. (D) Expression of inflammatory cytokines and chemokines in AC16 with or without silencing of IL-36R in vitro. The t test was used for comparison of 2 groups. All in vitro experiments were repeated 5 times. P<0.05 indicates a significant difference. * P<0.05, ** P<0.01.