(A-C) Sections from the outer medulla of kidneys from THP+/+ and THP−/− mice were stained for phosphorylated (active) c-JUN (p-C-JUN, A). Volumetric tissue exploration and analysis (VTEA) was used to quantify the percentage of cells positive for p-c-JUN staining (B). In C, 3D rendering of a representative p-c-JUN stained image is overlaid with the VTEA gating.
Scatter plot error bars represent mean ± standard deviation, * denotes (p< 0.05), ** denotes (p<0.01). M-OS = Medulla, outer stripe. CX = Cortex.