Table 7. Effect of biflavonoid on the activities of enzymic antioxidant in the liver of control and experimental rats.
| Groups | SOD | Catalase | Glutathione peroxidase | Glutathione-S- transferase |
|---|---|---|---|---|
| Control | 9.04 ± 0.80 | 81.93 ± 7.62 | 7.72 ± 0.76 | 9.17 ± 0.75 |
| Normal + Biflavonoid (80mg/kg b w) | 10.35 ± 0.68 | 84.14 ± 7.58 | 8.43 ± 0.79 | 9.84 ± 0.82 |
| Diabetes Induced | 3.76 ± 0.34b | 54.42 ± 3.49b | 3.49 ± 0.30b | 4.26 ± 0.38b |
| Diabetes + Biflavonoid (80mg/kg b w) | 8.06 ± 0.66c | 73.12 ± 6.52c | 6.41 ± 0.52c | 7.60 ± 0.53c |
| Diabetes + Metformin (500mg/kg b w) | 8.76 ± 0.63 | 75.02 ± 6.40 | 7.10 ± 0.65 | 8.33 ± 0.57 |
Values are considered significantly different at P < 0.05 with post hoc LSD test *P < 0.05.
The symbols a, b c and d also represent the statistical significance at p < 0.05.
a Control vs Drug control rats(normal rats received biflavonoid alone).
b Control vs Diabetic rats.
c Diabetic control rats.vs Diabetic rats treated with biflavonoid (80mg/kg).
d Diabetic rats treated with biflavonoid (80 mg/kg) vs Diabetic rats treated with Metformin (500 mg/kg).
The activities of enzymes are expressed as follows: SOD – One unit of activity was taken as the enzyme quantity, which gave 50% inhibition of nitroblue tetrazolium reduction in 1 minute/mg protein; CAT – µmoles of H2O2 consumed/minute; GPx – µg of glutathione consumed/minute/mg protein; GST- µmoles of 1-chloro 2,4-dinitrobenzene-GSH conjugate formed/minute/mg protein.