Table 2.

Evaluation of putative cross-linking reagents by using selected nucleic acid and protein-nucleic acid substrates (see Experimental). The production of inter-molecular crosslinks was assessed by using both denaturing PAGE and MS analysis. The observed performance was rated as excellent (✓ ✓ ✓), acceptable (✓ ✓), limited (✓), or negligible (✖).

Name	Class	DNA type 0	RNA type 0	Protein type 0	DNA-DNA crosslink	RNA-RNA crosslink	Prot.-RNA crosslink
8-methoxypsoralen	Psoralens	✓ ✓ ✓	✓	n.d.	✓ ✓ ✓	✖	✖
Trioxsalen	“	✓ ✓	✓	n.d.	n.d.	✖	n.d.
4′-aminomethyltrioxsalen hydrochloride	“	✓ ✓ ✓	✓ ✓ ✓	n.d.	✖	✖	✖
Transplatin	Platinum complexes	✓ ✓ ✓	✓ ✓ ✓	✓ ✓	✓ ✓ ✓	✓ ✓ ✓	✓ ✓ ✓
Cisplatin	“	✓ ✓ ✓	✓ ✓ ✓	✓ ✓	✓ ✓ ✓	✓ ✓ ✓	✓
trichloro(ethylene) platinate(II)	“	n.d.	✓ ✓ ✓	n.d.	n.d.	✓ ✓ ✓	✓ ✓ ✓
Tetrachloro platin	“	n.d.	✓ ✓ ✓	✓ ✓	n.d.	✓ ✓ ✓	✓ ✓ ✓
Nitrogen mustard	Mustard	n.d.	✓ ✓ ✓	✓ ✓	n.d.	✓ ✓ ✓	✓ ✓ ✓
Chlorambucil	“	n.d.	✓ ✓ ✓	n.d.	n.d.	✓ ✓ ✓	✓ ✓
Carmustine	“	n.d.	✓ ✓	n.d.	n.d.	✓ ✓	✓ ✓ ✓
Bis(2-chloroethyl)amine	Alkylamine	n.d.	✓ ✓	✓ ✓	n.d.	n.d.	✓ ✓
Bis(2-iodoethyl)amine	“	n.d.	✓ ✓	✓ ✓	n.d.	n.d.	✓ ✓
Epibromo hydrin	epoxydes	n.d.	✖	✖	n.d.	✖	✖
1,4 butanediol diglycidyl ether	“	n.d.	✖	✖	n.d.	✖	✖
Busulphan	Alkyl sulphonate	n.d.	✖	✖	n.d.	n.d.	✖
Nitrous acid	Nitrous acid	n.d.	✖	✖	n.d.	n.d.	✖
Formaldehyde	aldehyde	n.d.	✓ ✓	✓ ✓	n.d.	n.d.	✓ ✓ ✓
Ethidium diazide	Photo-cross-linker	n.d.	n.d	n.d	n.d.	✖	✖
p-Azidophenyl isothiocyanate	“	n.d.	n.d	✓ ✓ ✓	n.d.	n.d.	✖
SDA (succinimidyl 4,4-azipentanoate)	“	n.d.	✖	✓ ✓ ✓	n.d.	✖	✖
succinimidyl-[4-(psoralen-8-yloxy)]-butyrate (SPB)	“	n.d.	n.d	✓ ✓ ✓	n.d.	✖	✖
2-iminothiolane	“	n.d.	✖	✓ ✓	n.d.	n.d.	✖
Bis(sulfosuccinimidyl) suberate	Activated carboxylic acid	n.d.	✖	✓ ✓ ✓	n.d.	✖	✖