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. 2020 Feb 14;8:1. doi: 10.3389/fchem.2020.00001

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Copyright © 2020 Huang, Huang, Zhang, Zhuang, Liu, Su, Ye, Xu, Kuang and Xie.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Representative flow cytometric plots showing CD45⁺CD3⁺ T cells in single live cell suspension of MC-38 tumors on days 3 and 8 after PTT. Flow cytometry results showing the percentage of CD45⁺CD3⁺ T cells (B), CD8⁺ tumor-infiltrating T cells (C), and the ratio of CD8 T cells to Tregs (D) on days 3 and 12 after PTT in distant MC-38 tumors. n = 5 in each group. Data are presented as means ± SEM. Statistical significance was calculated by Student's t-test: **P < 0.01, *P < 0.05.