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. 2020 Feb 14;13:1375–1396. doi: 10.2147/OTT.S236111

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© 2020 Song et al.

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Verification of lentiviral transfer efficiency for EFEMP2 knockdown in normal lung epithelial cells.

Notes: (A) Phase contrast images and (B) GFP fluorescence images showed effective lentiviral transfer efficiencies in normal lung epithelial cell line BEAS-2B, with more than 80% GFP positive cells. The expression of EFEMP2 in EFEMP2 shRNA transfected cells, negative control shRNA transfected cells and uninfected cells were measured by (C) RT-qPCR and (D) Western blotting (cropped blot). Magnification ×200. *P < 0.05.