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. 2020 Feb 14;13:1375–1396. doi: 10.2147/OTT.S236111

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© 2020 Song et al.

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Effects of EFEMP2 up- or down-regulation on lung normal and cancer cell proliferation and clonogenicity.

Notes: Cell proliferation ability was evaluated by growth curve, and cell clonogenicity ability was evaluated by plate clone formation assay. (A) EFEMP2 down-regulation distinctly promoted cell proliferative abilities of normal lung epithelial cell line BEAS-2B. (B) The colony numbers formed by EFEMP2 shRNA transfected BEAS-2B cells were much more than that formed by the negative control cells. There was no significant difference between the negative control shRNA transfected cells and the uninfected cells. (C) EFEMP2 up-regulation significantly inhibited cell proliferative abilities of both lung cancer cell lines H1299 and H1650. (D) The colony numbers were markedly down-regulated in EFEMP2 cDNA transfected H1299 and H1650 cells, compared to negative control groups. There was no significant difference between the negative control cDNA transfected cells and the uninfected cells. EFEMP2 knockdown evidently promoted normal lung cell proliferation and clonogenicity, meanwhile, EFEMP2 overexpression distinctly inhibited lung cancer cell proliferation and clonogenicity. *P < 0.05.