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. 2020 Feb 14;13:1375–1396. doi: 10.2147/OTT.S236111

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© 2020 Song et al.

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Effects of EFEMP2 up-regulation on the EMT-related genes and MMPs.

Notes: (A) By Western blotting (cropped blot), EMT hallmarks, including E-cadherin, N-cadherin, vimentin, Snail, Slug and Twist, were measured in control or EFEMP2 cDNA transfected H1299 and H1650 cells. (B) By RT-qPCR, EFEMP2 up-regulation increased the expression of E-cadherin, and decreased the expression of N-cadherin, Vimentin, Snail, Slug, Twist, MMP2 and MMP9, at mRNA levels. (C) By MMP zymography assay, the activities of MMP2 and MMP9 were significantly inhibited by EFEMP2 up-regulation. EFEMP2 knockdown could promote EMT and enhance the expression and activity of MMP2 and MMP9, while EFEMP2 overexpression could hamper the process of EMT and weaken the expression and activity of MMP2 and MMP9 in lung cancer. *P < 0.05.