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. 2020 Feb 21;15(2):e0229423. doi: 10.1371/journal.pone.0229423

Fig 2. RNA yield according to NanoDrop spectrophotometer (black bars) and 2100 Bioanalyzer (white bars) for three different extraction kits after storage of PBMCs in three different storage media.

Fig 2

A. Run 1. Storage of PBMCs for one day. RNA quantification after storage of RNA for 83 days. B. Run 2. Storage of PBMCs for 83 days. RNA quantification after storage of RNA for 127 days. Error bars represent the standard deviations of results from three independent extractions. Statistically significant differences are marked with one (p < 0.05) or two (p < 0.005) asterisks (Linear mixed model for repeated measures). EM: NucliSENS easyMAG; RE: RNeasy Mini Kit; RP: RiboPure RNA Purification Kit–blood; QZL: QIAzol; RNL: RNAlater; EML: easyMAG lysis buffer; RLT: RNeasy lysis buffer; RNL: RNAlater; RPL: RiboPure RNA Purification Kit–blood lysis buffer.