Table 2. RNA yield of different RNA extraction procedures according to NanoDrop and 2100 Bioanalyzer.
| RNA extraction procedure | NanoDrop (ND) | 2100 Bioanalyzer (BA) | ND vs. BA | |||
|---|---|---|---|---|---|---|
| RNA yield (ng) | RNA yield (ng) | p value | ||||
| Run 1 | Run 2 | Run 1 | Run 2 | Run 1 | Run 2 | |
| QZL-EM | 929.1 ± 72.2 | 1038.5 ± 111.7 | 385.0 ± 77.0 | 410.7 ± 117.6 | 0.020 | 0.037 |
| RNL-EM | 745.6 ± 69.1 | 667.3 ± 58.8 | 333.7 ± 160.3 | 410.7 ± 160.3 | 0.035 | 0.057 |
| EML-EM | 647.6 ± 89.9 | 714.3 ± 40.9 | 333.7 ± 117.6 | 410.7 ± 117.6 | 0.030 | 0.044 |
| QZL-RE | 1076.7 ± 42.3 | 1371.0 ± 186.5 | 700.0 ± 132.3 | 883.3 ± 251.7 | 0.020 | 0.015 |
| RNL-RE | 432.8 ± 92.0 | 296.7 ± 51.3 | 350.0 ± 50.0 | 300.0 ± 100.0 | 0.418 | 0.921 |
| RLT-RE | 913.3 ± 9.0 | 1006.2 ± 84.9 | 700.0 ± 50.0 | 883.3 ± 125.8 | 0.017 | 0.419 |
| QZL-RP | 1037.3 ± 61.4 | 2185.9 ± 178.5 | 548.2 ± 193.2 | 716.8 ± 193.2 | 0.072 | < 0.001 |
| RNL-RP | 1307.2 ± 160.7 | 1343.4 ± 90.7 | 885.5 ± 253.0 | 969.8 ± 386.5 | 0.036 | 0.171 |
| RPL-RP | 3299.5 ± 459.1 | 3634.8 ± 301.0 | 1560.2 ± 193.2 | 2066.2 ± 318.4 | 0.029 | 0.019 |
Results are means and standards deviation (in ng) from three independent extractions.
p values were calculated using the t test for paired samples.
Run 1. Storage of PBMCs for one day. RNA quantification after storage of RNA for 83 days
Run 2. Storage of PBMCs for 83 days. RNA quantification after storage of RNA for 127 days