Table 3. RNA quantification according to NanoDrop spectrophotometer, 2100 Bioanalyzer and RT-qPCR for the ACTB gene, for two runs of three different RNA extraction kits after storage of PBMCs in three different storage media.
| PBMC Storage medium | RNA extraction kit (elution volume; final volume (μl)) | NanoDrop | 2100 Bioanalyzer | RT-qPCR | |||
|---|---|---|---|---|---|---|---|
| RNA concentration (ng/μl)* | RNA concentration (ng/μl)* | Cq values● | |||||
| Run 1 | Run 2 | Run 1 | Run 2 | Run 1 | Run 2 | ||
| QIAzol | easyMAG (35; 77) | 12.1 ± 0.9 | 13.5 ± 1.5 | 5.0 ± 1.0 | 5.3 ± 1.5 | 29.1 ± 0.1 | 35.3 ± 0.5 |
| RNAlater p | 9.7 ± 0.9 | 8.7 ± 0.8 | 4.3 ± 2.1 | 5.3 ± 2.1 | 30.8 ± 1.0 | 33.7 ± 1.1 | |
| RNAlater s | 5.6 ± 0.9 | 6.3 ± 0.9 | 3.3 ± 1.5 | 7.3 ± 0.6 | 27.3 ± 0.2 | 29.7 ± 1.8 | |
| EML | 8.4 ± 1.2 | 9.3 ± 0.5 | 4.3 ± 1.5 | 5.3 ± 1.5 | 29.4 ± 0.4 | 31.4 ± 0.8 | |
| QIAzol | RNeasy (50; 50) | 21.5 ± 0.8 | 27.4 ± 3.7 | 14.0 ± 2.6 | 17.7 ± 5.0 | 27.7 ± 0.1 | 27.5 ± 0.5 |
| RNAlater p | 8.7 ± 1.8 | 5.9 ± 1.0 | 7.0 ± 1.0 | 6.0 ± 2.0 | 29.7 ± 0.5 | 31.1 ± 0.5 | |
| RNAlater s | 3.6 ± 1.3 | 3.1 ± 0.3 | 5.0 ± 1.7 | 3.7 ± 1.2 | > 40 | > 40 | |
| RLT | 18.3 ± 0.2 | 20.1 ± 1.7 | 14.0 ± 1.0 | 17.7 ± 2.5 | 27.5 ± 0.2 | 26.8 ± 0.3 | |
| QIAzol | RiboPure (100; 126.5) | 8.2 ± 0.5 | 17.3 ± 1.4 | 4.3 ± 1.5 | 5.7 ± 1.5 | 36.7 ± 0.1 | 31.0 ± 0.4 |
| RNAlater p | 10.3 ± 1.3 | 10.6 ± 0.7 | 7.0 ± 2.0 | 7.7 ± 3.1 | 31.4 ± 0.2 | 31.6 ± 0.4 | |
| RNAlater s | 2.3 ± 0.4 | 2.2 ± 0.5 | 2.3 ± 0.6 | 2.0 ± 1.0 | > 40 | > 40 | |
| RPL | 26.1 ± 3.6 | 28.7 ± 2.4 | 12.3 ± 1.5 | 16.3 ± 2.5 | 28.9 ± 0.6 | 29.4 ± 0.3 | |
*Run 1. Storage of PBMCs for one day. RNA quantification after storage of RNA for 83 days
Run 2. Storage of PBMCs for 83 days. RNA quantification after storage of RNA for 127 days
●Run 1. Storage of PBMCs for one day. RT-qPCR was performed after storage of RNA for 398 days
Run 2. Storage of PBMCs for 83 days. RT-qPCR was performed after storage of RNA for 442 days