KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Donkey anti-goat secondary antibody, alexa fluor 1488 | Invitrogen | Cat#A-11055; RRID:AB_142672 |
| Donkey anti-goat secondary antibody, alexa fluor 555 | Invitrogen | Cat#A-21432; RRID:AB_141788 |
| Donkey anti-goat secondary antibody, alexa fluor 647 | Invitrogen | Cat#A-21447; RRID:AB_141844 |
| Donkey anti-rat secondary antibody, alexa fluor 488 | Invitrogen | Cat#A-21208; RRID:AB_141709 |
| Donkey anti-rat secondary antibody, DyLight 550 | Invitrogen | Cat#SA5-10027 RRID:AB_2556607 |
| Donkey anti-rabbit secondary antibody, alexa fluor 555 | Invitrogen | Cat#A-31572; RRID:AB_162543 |
| Donkey anti-mouse secondary antibody, alexa fluor 647 | Invitrogen | Cat#A-31571; RRID:AB_162542 |
| APC anti-mouse CD31 monoclonal antibody | BioLegend | Clone#: MEC13.3; Cat#102510; RRID:AB_312917 |
| FITC anti-mouse CD31 monoclonal antibody | BioLegend | Clone#: MEC 13.3; Cat#102506, RRID:AB_312913 |
| CD31 monoclonal antibody, PE-Cyanine7 | eBioscience, Thermo Fisher Scientific | Clone#: 390; Cat#25-0311-81; RRID:AB_2734962 |
| APC anti-mouse CD45 monoclonal antibody | BioLegend | Clone#: 30-F11; Cat#103112; RRID:AB_312977 |
| FITC anti-mouse CD45 monoclonal antibody | BioLegend | Clone#: 30-F11; Cat#103108; RRID:AB_312973 |
| Pacific Blue anti-mouse Sca-1 monoclonal antibody | BioLegend | Clone#: D7 Cat#108120; RRID:AB_493273 |
| PE/Cy7 anti-mouse VCAM-1 monoclonal antibody | BioLegend | Clone#: 429 (MVCAM.A); Cat#105719; RRID:AB_2214047 |
| APC rat IgG2a, Isotype control monoclonal antibody | BioLegend | Clone#: RTK2758; Cat#400511 |
| FITC rat IgG2a, Isotype control monoclonal antibody | BioLegend | Clone#: RTK2758; Cat#400505; RRID:AB_2736919 |
| Pacific Blue rat IgG2a, Isotype control monoclonal antibody | BioLegend | Clone#: RTK2758; Cat#400527 |
| Mouse PDGF R alpha polyclonal antibody | R&D Systems | Cat#AF1062; RRID:AB_2236897 |
| Anti-Laminin 2 alpha monoclonal antibody | Abcam | Clone#: 4H8-2 Cat#ab11576, RRID:AB_298180 |
| Anti-Laminin polyclonal antibody | Sigma-Aldrich | Cat#L9393, RRID:AB_477163 |
| TCF4 polyclonal antibody | Novus | Cat#NBP1-88633 RRID:AB_11021504 |
| GAPDH monoclonal antibody | Novus | Cione:1D4 Cat#NB300-221; RRID:AB_10077627 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Tamoxifen | Sigma | Cat#T5648-5G |
| Endoxifen Hydrochloride Hydrate | Sigma | Cat#E8284-25MG |
| Polycaprolactone | Sigma-Aldrich | Cat#440752-250G |
| Methoxypolyethylene glycol 350 | Sigma-Aldrich | Cat#M6768-250G |
| BaCl2 | Sigma-Aldrich | Cat#B-0750 |
| Critical Commercial Assays | ||
| Standard Single-Cell West Kit | Protein Simple | K600 |
| Small Single-Cell West Kit | Protein Simple | K500 |
| Deposited Data | ||
| Tabula Muris Consortium Single Celi Droplet RNA Sequencing | Tabula Muris Consortium, 2018 | https://figshare.com/articies/Singie-ceii_RNA-seq_data_from_microfluidic_emuision_v2_/5968960 |
| Experimental Models: Cell Lines | ||
| Mouse 3T3 Fibroblasts | ATCC | CRL-1658 |
| Experimental Models: Organisms/Strains | ||
| PDGFRαCreER/+ | This paper | NA |
| R26NG | See derivation in methods; Yamamoto et al., 2009 | NA |
| R26NZG | The Jackson Laboratory; Yamamoto et al., 2009 | Jax stock # 012429 |
| R26Flp | The Jackson Laboratory; Farley et al., 2000 | Jax stock # 009086 |
| R26DTA | The Jackson Laboratory; Voehringer et al., 2008 | Jax stock # 009669 |
| NSG | The Jackson Laboratory; Shultz et al., 2005 | Jax stock # 005557 |
| UBC-GFP | The Jackson Laboratory; Schaefer et al., 2001 | Jax stock # 004353 |
| Oligonucleotides | ||
| Primer sequences | See Table S1 | N/A |
| SMARTpool: ON-TARGETplus Mouse Tcf4 siRNA | Dharmacon | #L-043160-02 |
| SMARTpool: ON-TARGETplus Non-targeting siRNA | Dharmacon | #D-001810-10 |
| Software and Algorithms | ||
| R software package Seurat | Butler et al., 2018 | https://satijalab.org/seurat/ |
| BD FACSDiva 8.0 | BD Biosciences | N/A |
| BZ-X Viewer version 01.03.00.05 | Keyence Corporation | N/A |
| BZ-X Analyzer version 1.3.0.3 | Keyence Corporation | N/A |
| Improvision Volocity 6.3.0 | Perkin Elmer | N/A |
| QDR 12 | Hologic, Inc | N/A |
| Scout 2.0.5 | ProteinSimple | https://www.proteinsimple.com/scout/downloads/ |
| GIMP 2.8 | N/A | https://www.gimp.org |
| ImageJ | N/A | https://imagej.nih.gov/ij/ |
| Prism 7 | GraphPad Software, Inc | N/A |