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. 2020 Feb 21;6(8):eaay3178. doi: 10.1126/sciadv.aay3178

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Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).

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Fig. 2 — (A) ¹H-¹⁵N HSQC of ΔN117 and ΔN165 in the 1:1 complex with cognate DNA. Assignment of the ΔN165 spectrum was 90% complete. (B) DNA binding by ΔN165 and ΔN117 in 0.1 M and 0.05 M NaCl, showing the impact of the PEST domain on the cooperativity of 2:1 complex formation. Parametric values of the equilibrium dissociation constants are given in table S1.