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. 2020 Feb 21;6(8):eaay9669. doi: 10.1126/sciadv.aay9669

Fig. 5. Biochemical evaluation of ARN23765 effects.

Fig. 5

(A) Immunoblot detection of mutant CFTR in whole lysates derived from CFBE41o− cells treated with vehicle alone (DMSO), VX-809 (1 μM), or ARN23765 (1 nM) and at different time points following cycloheximide (CHX)–induced block of protein synthesis. (B) Quantification of mutant CFTR (band B and band C) half-life (n = 3). (C) Conformational stability of mutant CFTR evaluated as thermoaggregation propensity, determined in cell lysates of CFBE41o− cells treated with vehicle alone (DMSO), VX-809 (1 μM), or ARN23765 (1 nM), in comparison to WT-CFTR. (D) Quantification of soluble CFTR band C by densitometry, normalized by HSP90AB1 expression (n = 3). Symbols (B and D) represent statistical significance of ARN23765 versus DMSO (*), VX-809 versus DMSO (#), or ARN23765 versus VX-809 (§). One symbol, P < 0.05; two symbols, P < 0.01; and three symbols, P < 0.001 (ANOVA with Dunnett’s post hoc test).