Fig. 3. Analyzing metabolites in the brain via singlet states.

(A) Top to bottom: the ¹H NMR spectrum of ex vivo mouse brains (128 scans, spectrum is divided by 32) and the corresponding singlet-filtered NAA_β and Glx_γ spectra (128 scans, acquisition time ~3 min). In all the spectra, a polynomial pulse sequence was appended for water suppression. All experiments have been conducted at 7.05 T (300 MHz). The experimental parameters for the sequence are reported in table S4. The composition of the buffer is reported in the Supplementary Materials. (B) T₁ of the Glx signal and (C) T_S, displaying long-lived behavior.