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. Author manuscript; available in PMC: 2020 Aug 5.
Published in final edited form as: Nature. 2020 Feb 5;578(7795):455–460. doi: 10.1038/s41586-020-1974-9

Figure 2. SPEN localizes to the X chromosome immediately upon Xist upregulation and throughout the stages of XCI, but is dispensable for maintenance of X-linked gene silencing.

Figure 2

a, Images from combined HaloTag labeling of SPEN (green) and FISH for Xist RNA (red) in mESCs during a time course of Xist induction, and b, in NPCs. c, Schematic of the SPEN-degron experiment in NPCs. d, Cumulative distribution of transcript allelic ratios across the X chromosome (n=387 genes) after SPEN depletion in NPCs. e, Cumulative distribution and f, violin plot representation of transcript allelic ratio of escapees after SPEN depletion in NPCs (two-sided Wilcoxon signed-rank test, n=65, horizontal lines denote the median, box limits correspond to upper and lower quartiles). d, e and f, show averages of two independent NPC clones. a, b, These experiments were repeated at least twice with similar results.