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. Author manuscript; available in PMC: 2021 Feb 1.
Published in final edited form as: Mol Neurobiol. 2019 Nov 9;57(2):1217–1232. doi: 10.1007/s12035-019-01781-9

Fig. 1. Vax1 is required for SCN development.

Fig. 1

a Sagittal in situ hybridization images from Allen Brain Atlas showing expression of Vax1 in the adult SCN, scale bar represents 0.5 cm. Allen Mouse Brain Atlas (2004), image 18, Vax1-RP_090303_04_G09-sagittal, http://mouse.brain-map.org/experiment/siv?id=81655570&imageId=81622614&initImage=ish&coordSystem=pixel&x=7192.5&y=3784.5&z=1. b H&E staining on coronal brain sections of Vax1WT (WT) and Vax1KO mice at P0 showing absence of SCN morphology in Vax1KO (circled; x20). c Avp in situ hybridization in coronal brain sections at P0 in WT and Vax1KO embryos (circled; x20). d Sagittal in situ hybridization images from Allen Brain Atlas showing expression of Rax, Vax1, Lhx1, FoxD1, Rora, and Six3 in the developing mouse brain at the indicated embryonic (E) age, scale bar 1 mm. Allen Developing Mouse Brain Atlas (2008), E11.5, Rax_RP090724_05_F3-sagittal, image 7, http://developingmouse.brain-map.org/experiment/siv?id=100085519&imageId=101449838&initImage=ish&x=4120&y=3200&z=3; Vax1-RP_090707_04_E04-saggital, image 6, http://developingmouse.brain-map.org/experiment/siv?id=100085523&imageId=101449914&initImage=ish&x=3512&y=2936&z=3. E13.5, Lhx1-RP080807_03E10-sagittal, image 18, http://developingmouse.brain-map.org/experiment/siv?id=100031987&imageId=100689017&initImage=ish&x=3936&y=5496&z=3; Foxd1-RP_090724_06_H11-sagittal, image 14, http://developingmouse.brain-map.org/experiment/siv?id=100075832&imageId=101262091&initImage=ish&x=4240&y=5392&z=3; Vax1-RP-090303_04_G09-sagittal, image 14, http://developingmouse.brain-map.org/experiment/siv?id=100072475&imageId=101210859&initImage=ish&x=3616&y=5600&z=3. E15.5, Rora-RP_071203_02_H06-sagittal, image 16, http://developingmouse.brain-map.org/experiment/siv?id=100053213&imageId=101049221&initImage=ish&x=6144&y=7552&z=2; Six3-RP_080807_03_E12-sagittal, image 18, http://developingmouse.brain-map.org/experiment/siv?id=100032020&imageId=100689426&initImage=ish&x=3904&y=6488&z=2; Vax1-RP090303_04_G09-sagittal, image 14, http://developingmouse.brain-map.org/experiment/siv?id=100072496&imageId=101211230&initImage=ish&x=5152&y=7248&z=2 Abbreviations. OC: optic chiasm, SCN: suprachiasmatic nucleus, PVN: paraventricular nucleus, SON: supraoptic nucleus, Pit: pituitary.