WT and BMX-KO mice were subjected to CLP-induced sepsis surgery. (A) H&E staining of lung tissue after CLP at the indicated times. (B-E) Immunohistochemistry (IHC) for iNOS (B) and nitrotyrosine (D) with quantifications of % positive cells in (C) and (E). (F-K) Immunofluorescence analysis of the EC marker VE-cadherin (F), endothelial adherens junction marker CLDN5 (H) and the pericyte marker NG2 (J) in lung tissue sections after CLP at the indicated times with quantifications of % positive cells in (G), (I) and (J), respectively. Six mice in each group were used and quantified. Error bars represent the mean±SEM. ns: non significance; P < 0.05 were considered to indicate statistical significance between WT and BMX-KO mice at various time points using two-way ANOVA and Bonferroni post-hoc multiple comparisons. Scale bar: 20 μm (A,B,D,F,H,J).