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. 2020 Feb 21;3:82. doi: 10.1038/s42003-020-0808-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a–h Confocal images from L75Pfs organoids transduced with a control lentivirus carrying a pgkGFP expression cassette. GFP (c) was found in ARR3+ (b) cones; NRL (g) was not detected, and S-opsin+ cones (f) were localized throughout the ONL and no RHO (k) expression was detected, as expected in the absence of ectopic WT NRL expression. m–x Confocal images from L75Pfs organoids transduced with lentivirus carrying a pgkNRL expression cassette reveal patches of NRL expression within the ONL (o and s) and no NRL co-expression with S-opsin (n and r; merges in p and t). However, ectopically expressed NRL does co-express with RHO (q; merge in t), a rod marker that was never present in control transduced L75Pfs organoids. In pgkNRL-transduced organoids, RHO (w) did not co-localize with the cone marker ARR3 (v; merge in x). Scale bars = 25 μm.