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. 2020 Feb 21;10:3199. doi: 10.1038/s41598-020-60246-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The 3D-printed irradiation device and the experimental setup of cell irradiations. (A) The 3D-printed multi-step irradiation device filled with water. (B) The experimental setup with two 96-well plates (for clonogenics as the demonstration) placed on the top of the irradiation device. The plates can be irradiated with the large field of scanned protons from below. The arrows and texts in the images were edited using Snagit Editor.