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. 2020 Feb 21;10:3152. doi: 10.1038/s41598-020-60048-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Strain-specific reactivity of clones isolated from case 1 (A) and case 2 (B), presented as heatmap (upper figure). Each line in the heatmap represents the HA1 reactivity of clones produced by a single memory B-cell precursor cell. Heatmaps are generated by log2-transformation of the RFU and by applying hierarchical clustering, clusters are generated and indicated using different colors. The bar graph (below) represents the serum titer of both cases.