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. 2020 Feb 21;10:3156. doi: 10.1038/s41598-020-60145-9

Figure 3.

Figure 3

Time course observation of lumen formation in PLR123 stemCO and diffCO. (a,b) Bright-field (upper) or confocal fluorescence live-imaging of F-actin (lower) in stemCO (a) or diffCO (b) in PLR123 cells expressing LifeAct-RFP. (c,d) Whole mount immunostaining of Integrin α6 (ITGα6, green), phospho-Ezrin (p-Ezrin, red), phalloidin (white), and DAPI (blue) in stemCO (c) and diffCO (d). Scale bars = 100 μm (a–d).