Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Feb 21;10:3161. doi: 10.1038/s41598-020-59939-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Investigation of the chemoresistance function of miR-1229-3p in GC cells. (a) Overexpression of miR-1229-3p significantly induced the chemoresistance to 5-FU in HGC27 and GFP-MKN45 cells. (b) SLC22A7 as a novel target gene of miR-1229-3p in GC cells. An in silico search (http://www.targetscan.org/) identified SLC22A7 as a novel target gene of miR-1229-3p in GC. The seed regions of the miR-1229-3p and complementary SLC22A7 3′UTR sequences are presented in this figure. Overexpression of miR-1229-3p significantly reduced luciferase activity of the pmirGLO-SLC22A7 construct with cloned 3′UTR sequences of SLC22A7 in HGC27 and GFP-MKN45 cells. (c) MiR-1229-3p overexpression inhibited SLC22A7 mRNA and protein production. (d,e) TS and DPD of mRNA and protein levels were increased at 72 h after miR-1229-3p mimics transfection.