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. 2020 Feb 21;11:1004. doi: 10.1038/s41467-020-14815-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a, b CaPs (arrows) in Tg[SAIG213A] Tg[UAS:EGFP] fish. Orange, black and gray arrowheads indicate dorsal and ventral limits of the spinal cord, and ventral myotomal borders, respectively. c The structure of Tg[UAS:mRFP1-TDP-43z. d CaPs in Tg[SAIG213A] Tg[UAS:EGFP] (left) and Tg[SAIG213A] Tg[UAS:EGFP] Tg[UAS:mRFP1-TDP-43z] (right) larvae at 48 hpf. Arrows indicate CaPs expressing mRFP1-TDP-43z. e The lateral and frontal views of skeletonized CaP axons of Tg[SAIG213A] Tg[UAS:EGFP] (left) and Tg[SAIG213A] Tg[UAS:EGFP] Tg[UAS:mRFP1-TDP-43z] (right). The axon branch points and terminals are indicated by red and green, respectively. (See also Supplementary Movie 1). f, g Total length and branching frequency of CaP axons at the spinal segment 14–17 of Tg[SAIG213A] Tg[UAS:EGFP] (green, 12 cells, 5 animals) and Tg[SAIG213A] Tg[UAS:EGFP] Tg[UAS:mRFP1-TDP-43z] (magenta, 12 cells, 6 animals). *p < 0.0001 (unpaired t-test, two-tailed). NS, not statistically significant (p = 0.66). h Localization of mRFP1-TDP-43z in a CaP of Tg[SAIG213A] Tg[UAS:Gtuba2] Tg[UAS:mRFP1-TDP-43z] at 48 hpf. EGFP-tagged α-tubulin expressed from Tg[UAS:Gtuba2]⁶⁴ serves as a marker for the cytoplasm. The bars indicate 20 µm (a, b, d), 40 µm (e), and 2 µm (h). Error bars show standard deviation (SD).