Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Feb 21;10:3170. doi: 10.1038/s41598-020-60121-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Sanger sequencing chromatograms of a region in exon 6 of NAGLU gene from a Schipperke with MPS IIIB (forward and reverse) compared to the normal canine sequence (forward). The affected reverse sequence is flipped horizontally to align to the normal. The sequence traces show the poly-A/poly-T and loss of sequence quality, while trying to make it past the poly-A/poly-T insertion. An 11 bp region (boxed) is seen in both the forward and reverse sequences before the poly-A and poly-T respectively, demonstrating a duplication. The 704^th codon in the normal forward (CAG) and in the affected forward (CAA) at the junction of the insertion are underlined, it codes for glutamine in both (a synonymous variant). The affected sequence flanking the insertion deduced from the forward and reverse sequences is shown at the bottom of the figure.