Figure 2.

3D Fast Freeze Gelation, dissolution time and micro-morphology. (a) Freeze fixation; (b) Freeze gelation; (c) Scaffold detail displaying fluorescence spots. (d) Dissolution time of alginate ethanol gelled scaffold in DMEM, without (grey) or with (white) nanoparticles. (e) Alginate based scaffold (gelled with CaCl₂) after 24 h conservation in DMEM. (f) Alginate SEM images of 3D printing scaffold gelled with CaCl₂ and with (g) ethanol at different magnifications. Unpaired t-test was performed and statistical significance was set at p < 0.05. ** vs without nanoparticles. Data are represented as mean ± SEM.