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. 2020 Feb 21;10:3205. doi: 10.1038/s41598-020-60196-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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VSMC Viability. (a) Calcein AM-loaded VSMC after scaffold solubilisation with (b) direct or (c) indirect method, gelled with EtOH (left panels) or CaCl₂ (right panels). VSMC viability (Live/Dead assay) after dissolving the scaffold, gelled with EtOH or CaCl₂, with (d) direct or (e) indirect method. Unpaired t-test was performed and statistical significance was set at p < 0.05. Data are represented as mean ± SEM.