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. 2020 Feb 21;11(2):141. doi: 10.1038/s41419-020-2343-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a HUVECs were exposed to shear stress for 12 h and then subjected to immunofluorescence staining. Endothelial cells were labeled with anti-CD31 antibody. LC3 puncta indicated that UF significantly increased the number of autophagosomes in comparison with DF. Representative images from at least three independent experiments are shown. b, c Western blot analysis was performed to show the expression of BECLIN, LC3II/ LC3I, and p62 under UF or DF. Numbers under the blots were mean ± SD of three biologically independent experiments, and the first lane (ST) was served as relative control. HUVEC cells were treated with UF (d) or (e) with autophagy inhibitors, real-time PCR was performed to determine the expression of ICAM1, MCP-1, and KLF2. Data represent mean ± SD of three independent experiments (**P < 0.01, *P < 0.05, vs. the control).