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. 2020 Feb 21;11(2):141. doi: 10.1038/s41419-020-2343-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a After DF treatment together with 100 nM rapamycin administration for 12 h, western blot analysis was performed to show the expression of BECLIN 1, LC3II/ LC3I, and p62. Numbers under the blots were mean ± SD of three biologically independent experiments, and the first lane (Mock) was served as relative control. b Immunofluorescence staining showed the total abundance and nuclear enrichment of YAP protein in the presence of 100 nM rapamycin for 12 h. c Luciferase assay showed the activity of 8 × GTIIC reporter in HUVECs exposed to 100 nM of rapamycin treatment under DF for 12 h, and d real-time PCR showed the expression levels of YAP downstream genes CTGF and CYR61. e Immunofluorescence assay showed that chloroquine treatment enhanced YAP staining in the cytoplasm under UF. f Luciferase assay showed the activity of 8 × GTIIC reporter in HUVECs exposed to chloroquine under UF. Data represent mean ± SD of three independent experiments (**P < 0.01, *P < 0.05, vs. the control).