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. 2020 Feb 21;11(2):141. doi: 10.1038/s41419-020-2343-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a HUVECs were infected with lentivirus overexpressing SIRT1 for 72 h and the total abundance and nuclear enrichment of YAP protein in the presence of SIRT1 overexpression was detected using western blot. N-YAP represented nuclear YAP. Numbers under the blots were mean ± SD of three biologically independent experiments, and the first lane was served as relative control. b Luciferase assay showed the activity of 8 × GTIIC reporter in SIRT1-overexpressing HUVECs exposed to DF. Upper panel, western blot to confirm the SIRT1 level after overexpression. c Real-time PCR showed the expression levels of YAP downstream genes CTGF and CYR61 in SIRT1-overexpressing HUVECs. d HUVECs were treated with 10 μM sirtinol and exposed to UF. Then, western blot showed the total abundance of YAP protein under UF. e Real-time PCR showed the expression levels of YAP downstream genes CTGF and CYR61 under the treatment of 10 μM sirtinol together with UF.