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. 2020 Feb 21;11(2):141. doi: 10.1038/s41419-020-2343-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a Oil Red O staining of mice aortas in the ApoE^−/− mice fed a diet supplemented with cholesterol (Vehicle group) or with cholesterol plus Rapamycin (Rapamycin) for 8 weeks, and yellow dashed line indicates the size of plaque area as a percentage of total area. b Quantification of plaque size, Oil Red O-positive area in Vehicle group (n = 10) and rapamycin group (n = 10). Data are mean ± SEM. c Immunofluorescence staining for YAP and SIRT1 proteins in the atherosclerotic vessels from the vehicle group and rapamycin group. Nuclei are counterstained with DAPI (blue). White frame indicates endothelia membrane. Representative images are shown, n = 10. d Western blotting showed the total YAP protein level and SIRT1 level in tissues from vehicle group and rapamycin group. Three samples of each group were shown. Numbers under the blots were mean ± SD of three biologically independent experiments, and the first lane was served as relative control.