Figure 2.

LDIR accelerate zebrafish development in a VEGFR-dependent manner. Fli1:EGFP zebrafish larvae were exposed or not to 0.5 Gy at 3, 4 and 5 dpf, pre-treated or not with PTK/ZK, 30 minutes before each irradiation and photographed over-time. (A) Representative images of the vasculature from non-irradiated and irradiated zebrafish at the 27^th, 33^rd and 38^th dpf. The standard length (SL), in mm, was measured at each time-point for irradiated and non-irradiated zebrafish. (B–D) Post-embryonic development progress indicators were assessed at the 27^th and 33^rd dpf: (B) Head shape; (C) notochord flexion; (D) caudal fin; and (E) anal and dorsal fin. and Scale bars, 1 mm (A), 500 μm (C,D). (F) At the 33^rd dpf, developmental stage was established by quantification of vascular caudal fin area, using ImageJ. Representative images of the median phenotypes are showed next to the graph. Scale bars, 500 μm. Data are represented as mean ± SEM and two-way ANOVA test was used to determine differences between experimental groups; ***P < 0.001.