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. 2020 Feb 21;10:3137. doi: 10.1038/s41598-020-60129-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Wound healing and blastema formation, triggering the regeneration process, are not affected by LDIR after amputation in zebrafish. The caudal fin of adult Fli1:EGFP zebrafish was amputated at mid-fin level and immediately exposed or not to 0.5 Gy for 3 consecutive days. (A) Caudal fin regeneration was followed over time, and regenerated area was quantified (in pixels) using ImageJ software. Data are represented as mean ± SEM and two-way with repeated measures ANOVA test was used to determine differences between experimental groups; ns, non-significant. (B) The mRNA expression levels of mmp9 (wound-healing marker) and msxb (blastema formation marker) were determined by qRT-PCR and normalized to elongation factor 1. Data represent the relative mRNA expression in irradiated tissue compared to non-irradiated ones. Data are represented as mean ± SEM of 12 independent zebrafish and differences between groups was assessed by independent samples T-test; ns, non-significant.