Table 1.
Data collection and refinement statistics.
| Parameter | Granulovirus | Lysozyme |
|---|---|---|
| Electron energy/wavelength | 200 kV/0.025 Å | 200 kV/0.025 Å |
| Dose (fluence) per crystal | 4.7 e−/Å2 | 2.6 e−/Å2 |
| Space group | I 2 3 | P 43 21 2 |
| Unit cell | ||
| a, b, c | 103.2, 103.2, 103.2 Å | 79.1, 79.1, 38 Å |
| α, β, γ | 90°, 90°, 90° | 90°, 90°, 90° |
| No. of hits/indexed lattices | 20,608/17,974 | 1,339/1,051 |
| Resolution range | 72.97–1.55 (1.61–1.55) Å | 55.93–1.80 (1.86–1.80) Å |
| Unique reflections | 25,971 (2,577) | 15,284 (927) |
| Multiplicity | 495 (70) | 26 (7) |
| Completeness (%) | 100 (100) | 77.7 (50.4) |
| Rsplit (%) | 8.7 (168.6) | 16.7 (28.3) |
| Mean I/σ(I) | 9.22 (0.71) | 6.72 (4.64) |
| CC1/2 | 1.00 (0.19) | 0.94 (0.22) |
| CC* | 1.00 (0.56) | 0.98 (0.60) |
| Wilson B-factor (Å2) | 12.3 | 9.59 |
| Reflections used in refinement | 26,635 (2,630) | 9,067 (569) |
| Reflections used for R-free | 1297 (149) | 436 (25) |
| Rwork (%) | 17.1 (32.6) | 27.1 (37.2) |
| Rfree (%) | 19.7 (35.5) | 31.6 (38.6) |
| Number of non-hydrogen atoms | 2,128 | 1,069 |
| Macromolecules | 2,038 | 1,009 |
| Solvent | 90 | 60 |
| RMS bonds/angles | 0.012 Å/1.13 Å | 0.003 Å/0.47° |
| Ramachandran favored (%) | 97.1 | 96.1 |
| Ramachandran allowed (%) | 2.90 | 3.94 |
| Ramachandran outliers (%) | 0.41 | 0.00 |
| Rotamer outliers (%) | 0.45 | 0.94 |
| Clashscore | 1.74 | 7.58 |
| Average B-factor | 19.3 | 9.5 |
| Macromolecules | 19.2 | 9.5 |
| Solvent | 20.4 | 9.9 |
| PDB-ID | 6S2O | 6S2N |
Highest-shell values are shown within parentheses.