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. 2020 Feb 21;10:3184. doi: 10.1038/s41598-020-60159-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Representative photomicrographs of hematoxylin-eosin-stained hippocampus sections (100x) of control and Pb²⁺ groups. (A) Control group, normal cells exhibited round-shaped and pale-stained nuclei. (B) Pb²⁺ group, dead cells exhibited a shrunken cytoplasm with pyknotic nuclei (black arrows). (C) Percentage of cellular damage in the hippocampal region of mice exposed to Pb²⁺. Values are expressed as the percentage of damaged cells counted in ten fields per area, in three slides per mouse. *P < 0.001 vs. control, T-test and Mann-Whitney test.