Table 1.
Effects of Pb2+ exposure on brain redox environment.
| 23 PND | ||||||||
|---|---|---|---|---|---|---|---|---|
| Hippocampus | Striatum | Cortex | Cerebellum | |||||
| C | Pb2+ | C | Pb2+ | C | Pb2+ | C | Pb2+ | |
| ROS (mg of DCF/mg of protein) | 0.33 + 0.03 | 0.57 + 0.05* | 0.85 + 0.08 | 1.46 + 0.08* | 1.30 + 0.09 | 1.52 + 0.03* | 0.79 + 0.06 | 1.20 + 0.07* |
| Lipid peroxidation (µmoles of MDA/mg of protein) | 0.037 + 0.001 | 0.077 + 0.008* | 0.042 + 0.004 | 0.069 + 0.001* | 0.026 + 0.002 | 0.036 + 0.001* | 0.021 + 0.001 | 0.046 + 0.004* |
| MTT (% vs. Control) | 100 + 5.1 | 65.77 + 4.77* | 100 + 4.2 | 68.75 + 4.75* | 100 + 5.0 | 48.05 + 5.55* | 100 + 8.4 | 60.11 + 5.61* |
| GSH (nmol/g of tissue) | 2689.72 + 84.72 | 2630.15 + 89.85 | 2485.13 + 184.87 | 2531.12 + 278.88 | 2541.32 + 51.32 | 2603.96 + 88.96 | 2742.99 + 52.99 | 2548.51 + 48.51* |
| 60 PND | ||||||||
| ROS (mg of DCF/mg of protein) | 0.86 + 0.084 | 0.93 + 0.068 | 1.22 + 0.203 | 1.69 + 0.31 | 0.35 + 0.017 | 0.27 + 0.051 | 0.21 + 0.011 | 0.19 + 0.021 |
| Lipid peroxidation (µmoles of MDA/mg of protein) | 0.42 + 0.057 | 0.737 + 0.143* | 0.464 + 0.071 | 0.7 + 0.1 | 0.26 + 0.028 | 0.25 + 0.055 | 0.110 + 0.01 | 0.25 + 0.07* |
| MTT (% vs. Control) | 100 + 4.2 | 50.76 + 5.74* | 100 + 16 | 46.54 + 3.46* | 100 + 4.1 | 53.38 + 3.62* | 100 + 7.2 | 75.76 + 5.24* |
| GSH (nmol/g of tissue) | 2583.66 + 111.34 | 2573.11 + 131.89 | 2146.92 + 233.08 | 2237.48 + 137.52 | 2323.63 + 66.37 | 2246.58 + 153.42 | 2668.05 + 91.95 | 2256.63 + 83.37* |
Data were obtained in different cerebral regions: hippocampus, striatum, cortex and cerebellum at 23 and 60 PND. Data are mean ± S.E.M. T-test and Mann-Whitney test were used for comparisons, *P < 0.01 vs. each respective brain region control, n = 5–8 per group.