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. 2020 Jan-Mar;12(1):44–51.

Figure 1.

Figure 1.

A) Representative gel retardation assay of HR9 peptide complexed with pEGFP-NS3 at different N/P ratios: Lane 1: naked plasmid DNA as a control (pEGFP-NS3), Lane 2: N/P = 1:1, Lane 3: N/P=2:1, Lane 4: N/P = 5:1, and Lane 5: N/P = 10:1; B) Gel retardation assay of +36 GFP complexed with pEGFP-NS3 at various N/P ratios: Lane 1: pEGFP-NS3, Lane 2: N/P = 1:1, Lane 3: N/P = 2:1, Lane 4: N/P=5:1, Lane 5: N/P = 10:1 and Lane 6: N/P = 20:1. The mixtures were analyzed by electrophoresis on a 1% agarose gel. The DNAs complexed with HR9 peptide and +36 GFP that were not able to migrate into the gels were observed at an N/P ratio of 2:1 and 5:1, respectively.