Table 3.

The single tube OE- PCR reaction for construction of 5′UTR+ K2-SOX2/C-MYC/LIN28A-3′UTR

Step	Cycle	Denaturation	Annealing	Extension
1	1	95°C for 4 min	--	--
2	13	95°C for 30 s	X°C for 55 s	72°C for 3 min
3	1	50°C for 6 min	--	--
4	32	95°C for 30 s	55°C for 50 s	72°C for 3 min
5	1	--	--	72°C 5 min