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. 2020 Feb 5;117(7):3610–3620. doi: 10.1073/pnas.1904469117

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Copyright © 2020 the Author(s). Published by PNAS.

This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND).

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Fig. 3. — Delivery of tRNA to near-cognate and noncognate ribosomal complexes. (A) Structural representations depicting the approximate locations of P-site tRNA (orange), A-site tRNA (white), S12 (light yellow), and EF-Tu (red) during tRNA selection. The locations of Cy3 (green circle), LD650 (red circle), and LD750 (brown circle) are indicated. (B) Representative LD650 FRET (purple) and LD750 FRET (fuchsia) trajectories observed at 25-ms time resolution during noncognate tRNA selection (Left), near-cognate selection (Center), and cognate selection (Right) in S12-labeled complexes at 15 mM Mg²⁺; individual fluorescence traces for each dye channel are not shown for clarity of presentation. (C) Population LD650 FRET histograms (Upper) and corresponding LD750 histograms (Lower) observed in noncognate (Left), near-cognate (Center), and cognate (Right) tRNA selection.