Fig. 4.

Nanoparticle size, surface chemistry, and multiplexed administration dictate circulation half-life and biodistribution. (A) Schematic of PLGA and PLA-PEG polymeric nanoparticles encapsulating fluorescent dye. Circulation half-life measurements following IV administration of (B) DiD-loaded PLGA nanoparticles and (C) DiD-loaded PLA-PEG nanoparticles in male (light blue) and female (light red) mice (n = 3 mice per group per time point), and in a multiplexed experiment (dark blue) where mice were simultaneously administered DiI-loaded PLA-PEG and DiD-loaded PLGA NPs using quantitative microscopy (n = 3 mice per group per time point). Error bars represent SEM.