Table 1.

Recombinant Viruses

Virus name	C17L	C16L	C12	B22R	B23R	Insert	MRC-5*	A549†
v51.2	+	+	+	+	+	none	+++	+++
V51.2ΔC17/B23	mCherry¶	+	+	+	mCherry	none	+++	+++
V51.2ΔC16/B22	+	GFP	+	GFP	+	none	++	+
V51.2ΔC17ΔC16	mCherry	mCherry	+	mCherry	mCherry	none	++	+
V51.2ΔC12	+	+	GFP	+	+	none	+	++
MVA	FS‡	truncated	−	Δ45 bp	FS	none	−	−
MVA+C17	FS	truncated	−	Δ45 bp	FS	C17L	−	−
MVA+C16	FS	truncated	−	Δ45 bp	FS	C16L	+	++
MVA+B22§	FS	truncated	−	repaired	FS	none	+	++
MVA+C16/C17	FS	truncated	−	Δ45 bp	FS	C16L+C17L	+	++
MVA+C12	FS	truncated	−	Δ45 bp	FS	C12L	++	+
MVA+C12/C16	FS	truncated	−	Δ45 bp	FS	C16L+C12L	+++	+++

^*Replication in MRC-5 cells. −, +, ++, and +++ indicate no, low, moderate, and high replication, respectively.

^†Replication in A549 cells. −, +, ++, and +++ indicate no, low, moderate, and high replication, respectively.

^‡Frame-shift.

^§B22R repaired by homologous recombination.

^¶mCherry or GFP replaced indicated ORF.