Table 2.
RNA primer volume.
| Components | Volume | Final concentration |
|---|---|---|
| Total RNA | 2 μg | 2 μg/rxn |
| Oligo dT primer (10 μM) | 1 μl | 0.5 μM |
| dNTPs (10 mM each) | 1 μl | 500 μM |
| RNase-free water | Up to 14.5 μl |
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