Table 3.
Reverse transcription reaction solution.
| Reagent components | Volume | Final concentration |
|---|---|---|
| Inhibitor (40 U/μl) | (20 U) | 20 U/rxn |
| 5X RT buffer | 4 μl | 1X |
| EasyScriptTMRTase (200 U/μl) | 1 μl (200 U) | 20 U/rxn |
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